Effects of IGF-1 on neural differentiation of human umbilical cord derived mesenchymal stem cells

详细信息    查看全文

• 作者：Lei Zhao^a ; Yetong Feng^b ; ^{fengyt11@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Xin Chen^c ; Jing Yuan^a ; Xiaobo Liu^d ; ^e ; Yaoyu Chen^d ; ^e ; Yifan Zhao^d ; ^e ; Pengfei Liu^d ; ^e ; ^{rockman123456@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Yalan Li^f ; ^{tyalan@jnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Umbilical cord derived mesenchymal stem cells ; Insulin-like growth factor-1 ; Neural differentiation ; Cell proliferation
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：151
• 期：Complete
• 页码：93-101
• 全文大小：1812 K

文摘

Umbilical cord derived mesenchymal stem cells (UC-MSCs) have been demonstrated to hold the potential to be applied in the treatment of kinds of disease. In recent years, some scientists have differentiated the cells into neural progenitor cells (NPCs) successfully, providing a new cell source for neural disease therapy. However, the differentiation methods still need to be improved for the clinical studies in the future. In this study, insulin-like growth factor-1 (IGF-1) was tested to ameliorate UC-MSCs neural differentiation.

Main methods

IGF-1 overexpressing UC-MSCs (UC-MSCs-IGF-1) were established through retroviral infection, and further differentiated into NPCs through neural induction. The proliferation and differentiation ability of UC-MSCs derived NPCs were evaluated respectively and the associated signaling mechanisms were further analyzed with RNA microarray, qPCR and western-blot.

Key findings

Compared with NPCs from normal UC-MSCs, the NPCs derived from UC-MSCs-IGF-1 hold better proliferation ability and more Pax6-positive cells and Nestin-positive cells. Moreover, the UC-MSCs-IGF-1 derived NPCs could differentiate into astrocyte with higher efficiency during the process of terminal differentiation in vitro. RNA microarray analysis indicated that some key genes associated with neural differentiation and NPCs proliferation were upregulated, which were also confirmed with qPCR and western-blot. Finally, NPCs from UC-MSCs-IGF-1 transfected with IGF-1-siRNA showed a decrease of proliferation ability and astrocyte differentiation.

Significance

This study indicated that IGF-1 could improve neural differentiation of human UC-MSCs and provided a novel strategy to enhance astrocyte differentiation of NPCs from UC-MSCs.