Amelioratory effect of flavonoids rich Pergularia daemia extract against CFA induced arthritic rats

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• 作者：Devanesan Arul Ananth^a ; Angappan Rameshkumar^a ; ^b ; Ramachandran Jeyadevi^a ; G. Smilin Bell Aseervatham^a ; Jaganathan Sripriya^c ; Prabaharan Chandra Bose^d ; Thilagar Sivasudha^a ; ^{sudacoli@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{sudha@bdu.ac.in" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Rheumatoid arthritis ; Anti-inflammatory ; Flavonoids ; CFA ; LC-ESI/MS
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：80
• 期：Complete
• 页码：244-252
• 全文大小：2030 K

文摘

Pergularia daemia Forsk. (Asclepiadaceae) is a traditionally reported medicinal herb used to treat joint pain and arthritis. However, there are no scientific reports about anti-arthritic activity of P. daemia methanolic extract on rats as animal model. This study identifies bioactive compounds present in the P. daemia methanolic extract and evaluates its anti-arthritic potential in CFA induced arthritic rats.

Methods and results

Phytoconstituents of P. daemia extract were examined using LC-ESI/MS method. Anti-arthritic activity of P. daemia extract was determined by various biochemical experiments (RF, ESR and CRP), ultrasonography and histological analysis. LC-ESI/MS analysis resulted in the identification of major flavonoids compounds such as formononetin, qurecetin, chrysoeriol, taxifolin and naringenin. Serum biomarker analysis, after the treatment with PDME (500 mg/kg b.w.) revealed that the hemoglobin (11.84 ± 0.42 g/dL) and RBC (8.38 ± 0.67 million/mm³) levels were significantly increased whereas WBC (8.91 ± 0.38 thousands/mm³), RF (17.94 ± 0.45 IU/mL), ESR (7.91 ± 0.12 mm/h) and CRP (22.56 ± 0.26 mg/L) levels were decreased when compared with the CFA induced arthritic control group. Histology results revealed that treatment with PDME has resulted in significant prevention against bony destruction by decreasing soft tissue swelling and narrowing of joint spaces (250 and 500 mg/kg b.w.).

Conclusion

Anti-arthritic effect of P. daemia might be due to the presence of these bioactive flavonoids. These findings lend pharmacological support to the reported folkloric use of P. daemia in the treatment and management of painful, arthritic inflammatory conditions.