Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas as an alternative to Soxhlet and ultrasound-assisted extraction

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• 作者：Emanuela Martino ; Ilaria Ramaiola ; Mariangela Urbano ; Francesco Bracco and Simona Collina
• 关键词：Melilotus officinalis ; Coumarin ; HPLC analysis ; Extraction procedure ; Microwave-assisted extraction (MAE) ; Ultrasound-assisted extraction
• 刊名：Journal of Chromatography A
• 出版年：2006
• 出版时间：1 September 2006
• 年：2006
• 卷：1125
• 期：2
• 页码：147-151
• 全文大小：143 K

文摘

Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50 % v/v aqueous ethanol, two heating cycles of 5 min, 50 °C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.