Expression of IgH chain genes in antibody response to ragweed and purified Amb a 1: evidence for differential regulation of isotype production

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• 作者：Allauzen ; Sophie ; Blocki ; Frank ; Pierson-Mullany ; Lisa ; Jackola ; Duaine ; Dalan ; Dan ; Larue ; Cathryn ; et. al.
• 关键词：Amb a 1 ; group 1 protein from Ambrosia artemisiifolia ; w₁ ; short ragweed extract ; LCR-1 and LCR-2 ; locus control regions one and two ; PRIG ; polyreactive immunoglobulins ; RLU ; relative luminometer units ; mAb ; monoclonal antibody
• 刊名：Molecular Immunology
• 出版年：1999
• 出版时间：June, 1999
• 年：1999
• 卷：36
• 期：9
• 页码：587-598
• 全文大小：187 K

文摘

A survey of the work with Ig response to allergens carried out previously reveals an allergen-specific response both by IgE and all of IgG subclasses. Response of non-sensitive people is characterized by the appearance of a variety of the IgG subclasses. We have reexamined ragweed and Amb a 1 specific Ig response in 54 nonsensitive and 147 atopic or atopic-allergic people using a new inverse sandwich immunoassay allowing discrimination based on antibody affinity. We show that non-sensitive people present no, 0 out of 54, Ig response with affinities higher than K_a 10⁷ M⁻¹. The subpopulation of 66 atopics who never have experienced desensitization responds vigorously and solely (56 out of 66) with genes of the sequence γ2-α2. Only ten showed an additional weak response from γ1-α1. This suggests a possible association between the atopic state and selective activation of part of the gene sequence.