Pinacidil-postconditioning is equivalent to ischemic postconditioning in defeating cardiac ischemia-reperfusion injury in rat

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• 作者：Yi hui Yang^a ; ¹ ; ^{yihuiyangzy@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yu Zhang^b ; ¹ ; ^{gytzhangyu@163.com" class="auth_mail" title="E-mail the corresponding author} ; Wei Chen^a ; ^{weichenwhy@163.com" class="auth_mail" title="E-mail the corresponding author} ; Ying Wang^a ; ^{yingwangwhy@163.com" class="auth_mail" title="E-mail the corresponding author} ; Song Cao^b ; ^{songcaowhy@163.com" class="auth_mail" title="E-mail the corresponding author} ; Tian Yu^a ; ^b ; ^{dllyutian@163.com" class="auth_mail" title="E-mail the corresponding author} ; Haiying Wang^a ; ^{dllwanghaiyin@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Myocardial ; Ischemia-reperfusion injury ; Pinacidil ; Mitochondria ; Nrf2
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 June 2016
• 年：2016
• 卷：780
• 期：Complete
• 页码：26-32
• 全文大小：2256 K

文摘

Ischemic postconditioning (IPO) had been reported as a promising method against myocardial ischemia-reperfusion (I/R) injury, but IPO was later proved with poor clinical benefit. In this study, we compared the protective effects of pinacidil-postconditioning (PPO) and IPO against myocardial I/R injury. Langendorff rat hearts were randomly assigned to one of the following groups (n=8 each): Control group, I/R group (40 min ischemia and 60 min reperfusion), IPO group (6 successive cycles of 10 s reperfusion per 10 s occlusion before fully reperfusion), PPO group (perfused with 50 μM pinacidil for 5 min before reperfusion). Heart performance, infarct size and mitochondrial respiratory function were evaluated, and target genes/proteins of well-known Nuclear Factor-E2 Related Factor 2 (Nrf2) were assessed. Both IPO and PPO preserved heart function and myocardial ultrastructure at the end of reperfusion (all P<0.05 vs. I/R). The expression of Nrf2, NADH-quinone oxidoreductase-1 (NQO1), heme oxygenase 1 (HO-1) and superoxide dismutase 1 (SOD1) were similarly increased after IPO and PPO treatment (all P<0.05 vs. I/R). PPO exerted solid effect in defeating cardiac ischemia-reperfusion injury in rat.