Aberrant localization of lamin B receptor (LBR) in cellular senescence in human cells

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• 作者：Rumi Arai^a ; ¹ ; Atsuki En^a ; ¹ ; Ryo Ukekawa^a ; Kensuke Miki^a ; ^b ; Michihiko Fujii^a ; ^{mifuji@yokohama-cu.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Dai Ayusawa^a ; ^b
• 关键词：Cellular senescence ; Chromatin ; Nuclear envelope ; 5-Bromodeoxyuridine ; LBR
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：13 May 2016
• 年：2016
• 卷：473
• 期：4
• 页码：1078-1083
• 全文大小：1075 K

文摘

5-Bromodeoxyuridine (BrdU), a thymidine analogue, induces cellular senescence in mammalian cells. BrdU induces cellular senescence probably through the regulation of chromatin because BrdU destabilizes or disrupts nucleosome positioning and decondenses heterochromatin. Since heterochromatin is tethered to the nuclear periphery through the interaction with the nuclear envelope proteins, we examined the localization of the several nuclear envelope proteins such as lamins, lamin-interacting proteins, nuclear pore complex proteins, and nuclear transport proteins in senescent cells. We have shown here that lamin B receptor (LBR) showed a change in localization in both BrdU-induced and replicative senescent cells.