A novel visual loop-mediated isothermal amplification assay targeting gene62181533 for the detection of Salmonella spp. in foods
详细信息 查看全文
- 作者：Junjie Li ; Ligong Zhai ; Xiaomei Bie ; bxm43@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhaoxin Lu ; Xiaohan Kong ; Qian Yu ; Fengxia Lv ; Chong Zhang ; Haizhen Zhao
- 关键词：Gene62181533 ; Loop-mediated isothermal amplification ; Salmonella ; Visualization ; Primer combination
- 刊名：Food Control
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：60
- 期：Complete
- 页码：230-236
- 全文大小：1222 K
文摘
- •
A rapid and visual LAMP assay was developed for the detection of Salmonella.
- •
A non-opening cover effectively reduced cross-contamination in the assay.
- •
The assay required 50 min without expensive instruments.
- •
The assay targeted gene62181533, specific to Salmonella spp.
- •
The assay is highly sensitive (detection limit: 0.81 CFU/ml for pure cultures).
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |