Effects of deoxynivalenol on calcium homeostasis of concanavalin A—Stimulated splenic lymphocytes of chickens in vitro

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• 作者：Zhihua Ren^a ; ¹ ; ^{zhihua_ren@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yachao Wang^a ; ^b ; ¹ ; ^{wangyachao1688@126.com" class="auth_mail" title="E-mail the corresponding author} ; Huidan Deng^a ; ¹ ; ^{847767028@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Youtian Deng^a ; ^{335824070@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Junliang Deng^a ; ^{dengjl213@126.com" class="auth_mail" title="E-mail the corresponding author} ; Zhicai Zuo^a ; ^{zzcjl@126.com" class="auth_mail" title="E-mail the corresponding author} ; Ya Wang^a ; ^{wangyadudu@126.com" class="auth_mail" title="E-mail the corresponding author} ; Xi Peng^a ; ^{pengxi197313@163.com" class="auth_mail" title="E-mail the corresponding author} ; Hengmin Cui^a ; ^{cui580420@sicau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Liuhong Shen^a ; ^{shenliuhong@sohu.com" class="auth_mail" title="E-mail the corresponding author} ; Shumin Yu^a ; ^{yayushumin@163.com" class="auth_mail" title="E-mail the corresponding author} ; Suizhong Cao^a ; ^{caosuizhong@yahoo.com.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Deoxynivalenol ; Calcium homeostasis ; ATPase ; CaM ; Mycotoxin
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：68
• 期：4
• 页码：241-245
• 全文大小：882 K

文摘

In this study, the in vitro effects of the treatment of concanavalin A (Con A)—stimulated splenic lymphocytes with DON were examined. Splenic lymphocytes isolated from chickens were stimulated with 12.5 μg/mL Con A and exposed to deoxynivalenol (DON) (0–50 μg/mL) for 48 h. The intracellular calcium concentration ([Ca²⁺]i), pH, calmodulin (CaM) mRNA levels, and Na⁺,K⁺-ATPase and Ca²⁺-ATPase activities were detected. With the DON exposure concentrations increased, the [Ca²⁺]i and CaM mRNA levels gradually increased in a dose-dependent manner, and all the evaluated conconcentrations affected ATPase activity to the same extent. There were significant differences (P < 0.05 or P < 0.01) between the treatment groups and the control group. These results indicate that an imbalance in calcium homeostasis and intracellular acidification are components of DON cytotoxicity in chicken lymphocytes.