This study highlights the potential of dentin conditioning with temporal-controlled dexamethasone releasing chitosan nanoparticle system (Dex-CSnp).

Conditioning of dentin with Dex-CSnp reversed the deleterious effects caused by endodontic irrigants on dentin.

Dentin conditioning with Dex-CSnp enhanced odontogenic differentiation potential of SCAP.

The proposed bioactive nanopolymeric system can be used for dentin conditioning to achieve spatial and temporal control in regenerative endodontics.