Effect of Xinfeng capsule on nuclear factor kappa B/tumor necrosis factor alpha and transforming growth factor beta 1/Smads pathways in rats with cardiac injuries induced by adjuvant arthritis

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• 作者：Huang Chuanbing^a ; Wan Lei^a ; Liu Jian^a ; ^{Iiujianahzy@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Arthritis ; Experimental ; Heart function tests ; Xinfeng capsule ; NF-kappa B ; Tumor necrosis factor-alpha ; Transforming growth factor-ß
• 刊名：Journal of Traditional Chinese Medicine
• 出版年：2016
• 出版时间：15 February 2016
• 年：2016
• 卷：36
• 期：1
• 页码：92-100
• 全文大小：1551 K

文摘

To investigate effects of Xinfeng capsule (XFC) on cardiac function in rats with adjuvant arthritis (AA) and explore the mechanism of these effects.

absSec_2">Methods

ara160">Forty-eight rats were randomly divided into normal control (NC), model control (MC), methotrexate (MTX) and XFC groups of equal size. In all groups except for the NC group, 0.1 mL Freund's complete adjuvant (FCA) was intracutaneously injected in the right rear vola pedis to induce inflammation. Drugs were applied beginning 19 days after induction of inflammation. Normal saline was administered to the NC and MC groups and 1 mg/100 g MTX (weekly) and 0.12 g/100 g XFC (daily) to the MTX and XFC groups, respectively. Rats were sacrificed after 30 day of treatment. Toe swelling degree (TSD), arthritis index (AI), cardiac function and expression of nuclear factor kappa B (NF-κB)/ tumor necrosis factor alpha (TNF-α) and transforming growth factor beta 1 (TGF-ß1)/Smads pathway proteins were measured.

absSec_3">Results

ara170">In the MC group, TSD and AI were greatly increased, while parameters of cardiac function were decreased and morphological analysis showed myocardial cell damage. Expression of TNF-α, NF-κB, Smad2, P-Smad2, Smad4 and TGF-ß1 proteins were elevated in cardiac tissue, while Smad7 expression was decreased. TSD and AI values closely correlated to parameters of cardiac function and to levels of proteins in the NF-κB/TNF-α and TGF-ß1/Smads pathways. Certain correlations were identified among TGF-ß1 and NF-κB, Smad2, P-Smad2 and Smad4. With XFC intervention, both TSD and AI were decreased and parameters of cardiac function and ultrastructure of myocardial cells improved. Expressions of NF-κB, Smad2, and Smad4 proteins were greatly decreased and Smad7 expression was elevated, as compared with levels in the MC and MTX groups.

absSec_4">Conclusion

ara180">XFC regulates expression of proteins in the NF-κB/TNF-α and TGF-ß1/Smads pathways, decreases immune complex deposition in cardiac tissue and improves cardiac function in AA rats via upregulation of Smad7.