A combination of two-state mechanism for trimethoprim and sulfamethoxazole determination was proposed.
The sensitivity could be improved 301 and 329 times for SMZ and TMP, respectively.
The limits of detection (LODs) were 8.5 ng/mL for TMP and 7.7 ng/mL for SMZ.
The LODs were lower than the maximum residue limits in foods of animal origin.
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