Modification of aspartokinase III and dihydrodipicolinate synthetase increases the production of l-lysine in Escherichia coli
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- 作者：Jianzhong Xua ; b ; Mei Hanb ; c ; Xidong Renb ; renxidong2012@sina.com" class="auth_mail" title="E-mail the corresponding author ; Weiguo Zhangb ; xujz126@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DAP ; diaminopimelate ; AEC ; S-(2-aminoethyl)-l-cysteine ; AEChr ; AEC high resistant ; Km ; kanamycin ; Rif ; rifampicin ; DCW ; dry cell weight ; LB ; Luria-Bertani ; LBG ; LB ; + ; 5 ; g ; L⿿1 glucose ; LBS ; LB ; + ; 100 ; g ; L⿿1 sucrose ; LBK ; LB ; + ; 50 ; μg ; mL⿿1⿿Km ; LBHIS ; LB ; + ; brain heart infusion ; + ; sorbitol ; α ; conversion rate of glucose
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：114
- 期：Complete
- 页码：79-86
- 全文大小：996 K
文摘
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Metabolic engineering E. coli LATR11 increases lysine production.
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AK III with mutation T344M shows non-feedback control and high-activity.
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DHDPS with mutation H56K shows non-feedback control and high-activity.
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Increasing flux through biosynthetic pathway benefits to improve lysine production.
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Heterologous expression of ddh in strain LATR11 benefits to increase productivity.
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