Characterization of 1,2-dibromoethane-degrading haloalkane dehalogenase from Bradyrhizobium japonicum

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• 作者：Christos C. Sfetsas ; Leonidas Milios ; Katholiki Skopelitou ; Anastasia Venieraki ; Rodanthi Todou ; Emmanouil Flemetakis ; Panagiotis Katinakis ; Nikolaos E. Labrou
• 关键词：Bioremediation ; 1 ; 2-Dibromoethane ; Haloalkane dehalogenase ; Hydrolase ; Rhizobial
• 刊名：Enzyme and Microbial Technology
• 出版年：2009
• 出版时间：5 November 2009
• 年：2009
• 卷：45
• 期：5
• 页码：397-404
• 全文大小：1372 K

文摘

Haloalkane dehalogenases (DHAs, E.C. 3.8.1.5) are very promising biocatalytic tools for the bioremediation of environmental pollutants which consists of haloalkanes. In the present work, we investigated the DHA from Bradyrhizobium japonicum USDA110 (BjDHA). The dehalogenase activity of B. japonicum USDA110 and RT-PCR analysis revealed that the BjDHA gene expression is induced by 1,2-dibromoethane (1,2-DBE) during the early exponential phase. The BjDHA gene was cloned, expressed in Escherichia coli BL21 (DE3) and characterized. The enzyme catalyzes the irreversible hydrolysis of a variety of haloalkanes to the corresponding alcohol, halide, and a hydrogen ion. The catalytic properties of the recombinant enzyme were investigated and the kinetic parameters (K_m, k_cat) for a number of substrates were determined. The results showed that the BjDHA displays wide substrate specificity towards haloalkanes and particular high activity towards 1,2-DBE. The enzyme has a different catalytic triad topology compared to the Xanthobacter haloalkane dehalogenase and is more similar to the Rhodococcus enzyme. In addition, consistent with its broad specificity, the BjDHA has a substantially larger and more polar active site cavity compared to the Xanthobacter and Rhodococcus enzymes and as a consequence, BjDHA is able to dehalogenate longer and polar compounds. These properties make this enzyme very promising bioremediation tool for environmental applications.