Coupling hybridization chain reaction with DNAzyme recycling for enzyme-free and dual amplified sensitive fluorescent detection of methyltransferase activity
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- 作者：Bingying Jianga ; jiangby@cqut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yulian Weib ; Junqiang Xua ; Ruo Yuanb ; Yun Xiangb ; yunatswu@swu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hybridization chain reaction ; DNAzyme ; Fluorescence ; Signal amplification ; DNA methyltransferase
- 刊名：Analytica Chimica Acta
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：949
- 期：Complete
- 页码：83-88
- 全文大小：1090 K
文摘
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A sensitive fluorescent method for detecting Dam MTase activity is developed.
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Signal amplification is achieved by the integration of HCR with DNAzyme recycling.
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The method is convenient and shows high sensitivity.
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The application of this method for anti-cancer drug screening is also demonstrated.
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