Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry

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• 作者：Fengxia Zhang ; Zhenhua Jia ; Peng Gao ; Hongwei Kong ; Xiang Li ; Jing Chen ; Qin Yang ; Peiyuan Yin ; Jiangshan Wang ; Xin Lu ; Famei Li ; Yiling Wu ; Guowang Xu
• 关键词：Atherosclerosis ; Metabonomics ; UFLC/MS-IT-TOF ; Plasma ; Urine ; Rats
• 刊名：Talanta
• 出版年：2009
• 出版时间：15 August 2009
• 年：2009
• 卷：79
• 期：3
• 页码：836-844
• 全文大小：924 K

文摘

An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass and MSⁿ fragments information obtained from UFLC/MS-IT-TOF analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. Concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-O-methyl-dopa, ethyl N2-acetyl-l-argininate, leucylproline, glucuronate, t6A N(6)-(N-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. Ursodeoxycholic acid, chenodeoxycholic acid, LPC (C16:0), LPC (C18:0) and LPC (C18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. The alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. This research proved that metabonomics is a promising tool for disease research.