The contribution of adenines in the catalytic core of 10-23 DNAzyme improved by the 6-amino group modifications

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• 作者：Junfei Zhu^a ; Zhiwen Li^a ; Qi Wang^b ; ^c ; Yang Liu^d ; Junlin He^b ; ^{hejunlin@bmi.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：10-23 DNAzyme ; Amino group ; Imidazolyl group ; Chemical modification ; Adenine
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：26
• 期：18
• 页码：4462-4465
• 全文大小：594 K

文摘

In the catalytic core of 10-23 DNAzyme, its five adenine residues are moderate conservative, but with highly conserved functional groups like 6-amino group and 7-nitrogen atom. It is this critical conservation that these two groups could be modified for better contribution. With 2′-deoxyadenosine analogues, several functional groups were introduced at the 6-amino group of the five adenine residues. 3-Aminopropyl substituent at 6-amino group of A15 resulted in a five-fold increase of k_obs. More efficient DNAzymes are expected by delicate design of the linkage and the external functional groups for this 6-amino group of A15. With this modification approach, other functional groups or residues could be optimized for 10-23 DNAzyme.