Efficient streptavidin-functionalized nitrogen-doped graphene for the development of highly sensitive electrochemical immunosensor

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• 作者：Zhanjun Yang ; ^{zjyang@yzu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Qingchun Lan ; Juan Li ; Jiajia Wu ; Yan Tang ; Xiaoya Hu ; ^{xyhu@yzu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nitrogen-doped graphene ; Streptavidin ; Carcinoembryonic antigen ; Electrochemical immunosensor
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：89
• 期：part_P1
• 页码：312-318
• 全文大小：910 K

文摘

In this work, an efficient and universal streptavidin-functionalized nitrogen-doped graphene (NG) was for the first time proposed and used to develop a highly sensitive electrochemical immunosensor for the detection of tumor markers. Transmission electron microscopy, electrochemical impedance spectrum, static water contact measurement, and cyclic voltammetry were used to characterize the streptavidin-functionalized NG platform and immunosensor. The biofunctionalized NG showed excellent hydrophilicity, larger specific surface area, and high electrochemical activity. These properties of the platform enhanced the loading capacity of proteins, and retained the bioactivity of the immobilized proteins, and thus remarkably improved the sensitivity of the immunosensor. Using carcinoembryonic antigen (CEA) as model analyte, the proposed immunosensor demonstrated a wide linear range of 0.02–12 ng mL⁻¹ with a low detection limit of 0.01 ng mL⁻¹. The CEA immunosensor could be applied to detect human serum samples with satisfactory results. The streptavidin-functionalized NG material provided an universal and promising platform for the electrochemical immunosensing applications.