Development of an immunochromatographic assay for the rapid detection of bromoxynil in water

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• 作者：Jiang Zhu ; Wenchao Chen ; Yitong Lu ; Guohua Cheng
• 关键词：Bromoxynil ; Gold immunochromatographic assay (GICA) ; Colloidal gold ; One-step strip test ; Pesticide residue
• 刊名：Environmental Pollution
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：156
• 期：1
• 页码：136-142
• 全文大小：363 K

文摘

A rapid immunochromatographic one-step strip test was developed to specifically determine bromoxynil in surface and drinking water by competitive inhibition with the nano colloidal gold-conjugated monoclonal antibody (mAb). Bromoxynil standard samples of 0.01–10 mg L⁻¹ in water were tested by this method and the visual limit was 0.06 mg L⁻¹. The assay only required 5 min and one-step by dispensing a drop of sample solution onto a strip. Parallel analysis of water samples with bromoxynil showed comparable results from one-step strip test and ELISA. Therefore, the one-step strip test is very useful as a screening method for qualitative detection of bromoxynil in water.