B cell positive cross-match not due to anti-HLA Class I antibodies and first kidney graft outcome

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• 作者：Loredana Praticò ; -Barbato ; Raffaele Conca ; Paola Magistroni ; Gianluca Leonardi ; Alice Oda ; Federica Rosati ; Ercolino Leone ; Maurizio Tacconella ; Stefano Roggero ; Giuseppe Paolo Segoloni ; Antonio A
• 关键词：B lymphocyte cross-match ; First kidney transplant ; Weak/low-titre anti-HLA Class I antibodies ; Anti-HLA Class II antibodies
• 刊名：Transplant Immunology
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：19
• 期：3-4
• 页码：238-243
• 全文大小：398 K

文摘

The effect of B cell cross-match (XM) was investigated in 680 first deceased-donor kidney transplants in a single centre from 1990 to 1999: 74 transplants presented a B-positive XM (Group 1) 606 had a B-negative XM (Group 2). The absence in Group 1 of weak/low-titre anti-HLA Class I antibodies was assured blocking anti-Class I reactivity by treating B cells with non-cytotoxic anti-ß2 microglobulin (αβ2 M) serum before XM.

Graft survivals up to 5 years were not significantly different; some differences were nevertheless observed: HLA-A,B,DR mismatches influenced graft outcome in Group 1: patients with 0–2 mismatches had better survival than patients with 3–4. When analysed according DR mismatch, patients with 1 mismatch had worse graft survival than well matched patients (p < 0.05). No significant difference depending on HLA match was observed in Group 2. Early acute rejection rate was similar in the Groups except the rejection episodes after one year: Group 1 had significantly more. 61/74 patients of Group 1 were retrospectively analysed for anti-HLA-DR,DQ reactivity: only 11/61 had anti-HLA-DR or DQ antibodies (3/11 were donor specific); graft survival and rejections were not significantly different in the patients with and without anti-HLA Class II antibodies.

Anti-donor B cell reactivity, at XM, once excluded the presence of weak/low-titre anti-HLA Class I antibodies, did not influence first kidney graft survival.