A smart fluorescence nanoprobe for the detection of cellular alkaline phosphatase activity and early osteogenic differentiation

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• 作者：Feng-Yi Cao ; BS ; Jin-Xuan Fan ; BS ; Yue Long ; BS ; Xuan Zeng ; PhD ; Xian-Zheng Zhang ; PhD ; ^{xz-zhang@whu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Fluorescence nanoprobe ; Stem cell ; Alkaline phosphatase ; Osteogenic differentiation
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：12
• 期：5
• 页码：1313-1322
• 全文大小：1669 K

文摘

In the past decades, biomaterials were designed to induce stem cell toward osteogenic differentiation. However, conventional methods for evaluation osteogenic differentiation all required a process of cell fixation or lysis, which induce waste of a large number of cells. In this study, a fluorescence nanoprobe was synthesized by combining phosphorylated fluoresceinamine isomer I (FLA) on the surface of mesoporous silica-coated superparamagnetic iron oxide (Fe₃O₄@mSiO₂) nanoparticles. In the presence of alkaline phosphatase (ALP), the phosphorylated FLA on the nanoprobe would be hydrolyzed, resulting in a fluorescence recovery of FLA. During early osteogenic differentiation, a high-level expression of cellular ALP was induced, which accelerated the hydrolysis of phosphorylated FLA, resulting in an enhancement of cellular fluorescence intensity. This fluorescence nanoprobe provides us a rapid and non-toxic method for the detection of cellular ALP activity and early osteogenic differentiation.