We selected 15 CpGs as menstrual blood-specific marker candidates using HumanMethylation450 BeadChip array data.

cg09696411 and cg18069290 in the SLC26A10 gene demonstrated high menstrual blood-specificity.

A previously reported multiplex methylation SNaPshot was modified to detect 9 CpGs, which include cg09696411 and cg18069290.

The resultant multiplex allowed positive identification of blood, saliva, semen, vaginal fluid and menstrual blood.

Menstrual bloods produced methylation profiles that vary with menstrual cycle and sample collection methods.