Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors
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- 作者：Matthias Bä ; ckera ; 1Author Vitae ; Claudia Kochc ; 1Author Vitae ; Sabine EibencAuthor Vitae ; Fania GeigercAuthor Vitae ; Fabian EbercAuthor Vitae ; Hartmut GliemanndAuthor Vitae ; Arshak Poghossiana ; bAuthor Vitae ; Christina Wegec ; christina.wege@bio.uni-stuttgart.de" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Michael J. Schö ; ninga ; b ; schoening@fh-aachen.de" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Tobacco mosaic virus ; Coat protein ; Enzyme nanocarrier ; Glucose biosensor ; Virus/biomolecule nanohybrid
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：716-722
- 全文大小：2104 K
文摘
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Electrochemical biosensors may profit from tobacco mosaic virus (TMV)-derived enzyme nanocarriers used as adapter templates.
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TMV nanotubes or coat protein (CP) aggregates on sensor chips enable efficient high-density coupling of active enzymes.
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Since TMV tubes and, to a lesser extent, CPs stabilize enzymes, sensor reusability and performance are further enhanced.
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The new layout yielded the first amperometric glucose sensors improved by TMV- or CP-exposure of glucose oxidase conjugates.
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