Dexamethasone provoked mitochondrial perturbations in thymus: Possible role of N-acetylglucosamine in restoration of mitochondrial function

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• 作者：Santhosh Kumar Venugopalan^a ; ^b ; ^{natu_sea@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Shanmugarajan T.S.^b ; Navaratnam V.^a ; ^c ; Mansor S.M.^a ; Ramanathan S.^a
• 关键词：DEX ; dexamethasone ; NAG ; N-acetylglucosamine ; Withanolide A ; WA ; ROO ; peroxyl radical ; LPO ; lipid peroxide ; MDA ; malondialdehyde ; GSH ; glutathione ; GST ; glutathione S-transferases ; GPx ; glutathione peroxidase ; GR ; glutathione reductase ; MnSOD ; mitochondrial antioxidant manganese superoxide dismutase ; α-KDH ; alpha ketoglutarate dehydrogenase ; SDH ; succinate dehydrogenase ; ICDH ; isocitrate dehydrogenase ; MDH ; malate dehydrogenase ; NADPH ; nicotinamide adenine dinucleotide phosphate ; ATP ; adenosine
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：83
• 期：Complete
• 页码：1485-1492
• 全文大小：2039 K

文摘

Thymus mitochondria play a crucial role in immune function. This study identifies the novel protective role of N-Acetylglucosamine (NAG) in dexamethasone (DEX) induced mitochondrial perturbations in mice thymus. Mice were induced with DEX (5 mg/kg) and treated with NAG i.p. (266 μg/kg, 400 μg/kg and 800 μg/kg) for 14 days, Withanolide A (800 μg/kg) has been used as positive control. Dose dependent treatment of NAG against DEX significantly restored the mitochondrial enzyme levels (ICDH, KDH, SDH and MDH) and elevated the mitochondrial glutathione antioxidants defense (GSH, SOD, GPX and GST) thus improving the ATP status which was confirmed by ultrastructural alterations in mitochondria and nucleus using TEM studies. Further histopathological studies also revealed that NAG attenuate DEX induced thymotoxicity. Finally, the study concludes that dose dependent treatment of NAG supports a potential role in preventing DEX induced thymotoxicity and NAG acts as a beneficial pharmacological intervention in the DEX induced thymic repercussions.