Oxidative stress and potential biomarkers in tomato seedlings subjected to soil lead contamination

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• 作者：Chengrun Wang ; Xiaorong Wang ; Yuan Tian ; Yingang Xue ; Xianghua Xu ; Yunxia Sui ; Hongxia Yu
• 关键词：Lead (Pb) ; Oxidative stress ; Electron paramagnetic resonance (EPR) ; Heat shock protein 70 (HSP70) ; Superoxide radical (O₂ ^; //www.sciencedirect.com/scidirimg/entities/rad"" alt=""radical dot"" title=""radical dot"" border=""0"">ȡ
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：71
• 期：3
• 页码：685-691
• 全文大小：362 K

文摘

Oxidative stress and defense response in leaves of tomato seedlings exposed to extraneous lead (Pb) at 0–500 mg kg⁻¹ soil for nearly 2 months were investigated. Superoxide radical (O₂⁻) was quantified by electron paramagnetic resonance (EPR). Results showed that levels of O₂⁻, malondialdehyde (MDA), carbonyl group and superoxide dismutase (SOD) increased with the increase of bioavailable Pb. The O₂⁻ level was well correlated with MDA, carbonyl groups and SOD activities, suggesting that O₂⁻ might be responsible for them. Intensities in two bands of SOD isoenzymes increased along with added Pb in treatments against control, implying that multigenic expression in SOD enzymes were activated to counteract O₂⁻ stress. Heat shock protein 70 (HSP70) was induced sensitively by soil Pb, which was to alleviate oxidative damage (i.e. increased carbonyl groups). The overall results indicated that HSP70 and O₂⁻ were the most sensitive parameters and the combination of them might be potential biomarkers of soil Pb contamination in tomato seedlings.