Therapeutic effects of naringin on degenerative human nucleus pulposus cells for discogenic low back pain

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• 作者：Nianhu Li ; MD ; PhD^a ; ^b ; Camden Whitaker ; MD^a ; Zhanwang Xu ; MD ; PhD^b ; Michael Heggeness ; MD ; PhD^a ; ^d ; Shang-You Yang ; MD ; PhD^a ; ^c ; ^{syang6@kumc.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Aggrecan ; Disc degeneration ; Intervertebral disc matrix ; Naringin ; Nucleus pulposus cells ; TNF-α
• 刊名：The Spine Journal
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：16
• 期：10
• 页码：1231-1237
• 全文大小：1271 K

文摘

Over half the population of the world will suffer from moderate or severe low back pain (LBP) during their life span. Studies have shown that naringin, a major flavonoid in grapefruit and an active compound extracted from a Chinese herbal medicine (Rhizoma Drynariae) possesses many pharmacological effects.

Purpose

The aim of this study was to evaluate the influence of naringin on the growth of degenerative human nucleus pulposus (NP) cells, and its repair effects on protein and gene expressions of the cells.

Study Design/Setting

This was an in vitro investigation of the human NP cells isolated from degenerated intervertebral discs that were interacted with various concentrated of naringin.

Method

This study was exempted by the institutional Human Subjects Committee-2, University of Kansas School of Medicine—Wichita. Degenerative human NP cells were isolated from intervertebral discs of patients with discogenic LBP and cultured at 37°C with 5% CO₂. The proliferation of NP cells was determined following treatment with various concentrations of naringin. The protein expressions of tumor necrosis factor-α (TNF-α) and Bone morphogenetic protein 2 (BMP-2) were tested using enzyme-linked immunosorbent assay. Aggrecan and type II collagen levels were measured by immunohistological staining. Further examination of the gene expression of aggrecan, Sox6, and MMP3 was performed after intervention with naringin for 3 days.

Results

The human NP cells were successfully propagated in culture and stained positive with toluidine blue staining. Naringin effectively enhanced the cell proliferation at an optimal concentration of 20 µg/mL. Naringin treatment resulted in significant inhibition of TNF-α, but elevated protein expressions of BMP-2, collagen II, and aggrecan. Naringin also increased disc matrix gene activity including aggrecan and Sox6, and decreased the gene expression of MMP3.

Conclusion

Naringin effectively promotes the proliferation of degenerative human NP cells and improves the recuperation of the cells from degeneration by increasing expression of aggrecan, BMP-2, and Sox6 while inhibiting the expression of TNF-α and MMP3. This study suggests that naringin may represent an alternative therapeutic agent for disc degeneration.