An immobilized liquid interface prevents device associated bacterial infection in vivo

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• 作者：Jiaxuan Chen^a ; ^b ; Caitlin Howell^b ; ^c ; Carolyn A. Haller^a ; ^b ; Madhukar S. Patel^a ; ^b ; ^d ; Perla Ayala^a ; ^b ; Katherine A. Moravec^a ; Erbin Dai^a ; Liying Liu^a ; Irini Sotiri^b ; ^c ; Michael Aizenberg^b ; Joanna Aizenberg^b ; ^c ; ^e ; ^f ; ^{jaiz@seas.harvard.edu" class="auth_mail" title="E-mail the corresponding author} ; Elliot L. Chaikof^a ; ^b ; ^g ; ^{echaikof@bidmc.harvard.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：SLIPS ; Perfluorocarbon liquids ; Polytetrafluoroethylene ; Implant ; Infection ; In vivo
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：80-92
• 全文大小：3624 K

文摘

Virtually all biomaterials are susceptible to biofilm formation and, as a consequence, device-associated infection. The concept of an immobilized liquid surface, termed slippery liquid-infused porous surfaces (SLIPS), represents a new framework for creating a stable, dynamic, omniphobic surface that displays ultralow adhesion and limits bacterial biofilm formation. A widely used biomaterial in clinical care, expanded polytetrafluoroethylene (ePTFE), infused with various perfluorocarbon liquids generated SLIPS surfaces that exhibited a 99% reduction in S. aureus adhesion with preservation of macrophage viability, phagocytosis, and bactericidal function. Notably, SLIPS modification of ePTFE prevents device infection after S. aureus challenge in vivo, while eliciting a significantly attenuated innate immune response. SLIPS-modified implants also decrease macrophage inflammatory cytokine expression in vitro, which likely contributed to the presence of a thinner fibrous capsule in the absence of bacterial challenge. SLIPS is an easily implementable technology that provides a promising approach to substantially reduce the risk of device infection and associated patient morbidity, as well as health care costs.