Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment

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• 作者：Yongjun Zheng¹ ; Shizhao Ji ; ¹ ; ^{shizhaoji@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Haibin Wu¹ ; Song Tian ; Yunqing Zhang ; Li Wang ; He Fang ; Pengfei Luo ; Xingtong Wang ; Xiaoyan Hu ; Shichu Xiao ; ^{huangzhuoxiao@sohu.com" class="auth_mail" title="E-mail the corresponding author} ; Zhaofan Xia ; ^{xiazhaofan_smmu@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Amnion ; Cryopreservation ; Wound healing ; Macrophages ; Neovascularization ; Dermal substitute
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：56-67
• 全文大小：3996 K

文摘

Approximately 25% of diabetic patients suffer from diabetic lower-extremity ulcer throughout their lives and 7%–20% of patients will eventually need an amputation despite standard care treatment. The development of new therapies to treat diabetic wounds is urgent. In this study, we used cryopreserved living micronized amnion (300–600 μm) to treat wounds in diabetic mice. Post-thaw micronized amnion retained high cell viability, as well as intact cell morphology and membrane structure. When transplanted onto the wounds of db/db mice, the cryopreserved living micronized amnion greatly promoted wound healing in diabetic mice mainly by secreting growth, inflammation, and chemotaxis-related factors that regulated macrophage migration and phenotype switch, recruited CD34⁺ progenitor cells, and increased neovascularization. In addition, the micronized amnion matrix can exist in the dermis and serve as a long-term dermal scaffold. These results demonstrated the potential of the cryopreserved living micronized amnion as a ready-to-use living dermal substitute that addresses multiple defective physiological processes of impaired wounds to treat diabetic ulcers and other chronic wounds in clinics.