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Comparative sperm ultrastructure of twelve leptodactylid frog species with insights into their phylogenetic relationships
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Sperm ultrastructure of representatives of Leptodactylidae subfamilies Leptodactylinae, Paratelmatobiinae and Leiuperinae are described.

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Data support the revalidation of Engystomops as a true taxon, distinct from Physalaemus.

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The tail of the spermatozoa of E. petersi and E. freibergi (Edentulus clade) (Leiuperinae) was similar to that of P. poecilogaster (Paratelmatobiinae).

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Sperm characteristics validate the allocation of A. marmorata into a genus distinct from Leptodactylus.

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The ultrastructural characteristics of the spermatozoa provide strong evidence of a phylogenetic signal useful for systematics of the anurans.

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