Effects of aqueous extracts from Panax ginseng and Hippophae rhamnoides on acute alcohol intoxication: An experimental study using mouse model

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• 作者：Da-Chao Wen^a ; ^b ; Xiao-Yu Hu^b ; ^{xiaoyuhu@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Yan-Yan Wang^b ; Jian-Xing Luo^b ; Wu Lin^a ; Ling-Yan Jia^a ; Xin-Yue Gong^a
• 关键词：AAI ; Acute alcohol intoxication ; TCM ; Traditional Chinese Medicine ; PG ; Panax ginseng ; HR ; Hippophae rhamnoides ; AEPH ; aqueous extracts from Panax ginseng and Hippophae rhamnoides ; SPF ; Specific pathogen free ; ADH ; alcohol dehydrogenase ; ALDH ; aldehyde dehydrogenase ; EO ; microsomal ethanol oxidase ; β-EP ; β-endorphin ; LENK ; leucine-enkephalin ; ELISA ; enzyme-linked&ndash ; immunosorbent serologic assay ; MTD ; metadoxine ; LAEPH ; low dose AEPH ; MAEPH ; medium dose AEPH ; HAEPH ; high dose AEPH
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 November 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：67-73
• 全文大小：1320 K

文摘

Acute alcohol intoxication (AAI) is a frequent emergency, but therapeutic drugs with superior efficacy and safety are lacking. Panax ginseng (PG) and Hippophae rhamnoides (HR) respectively has a wide application as a complementary therapeutic agent in China for the treatment of AAI and liver injury induced by alcohol. We investigated the effects of aqueous extracts from PG and HR (AEPH) on AAI mice and identified its underlying mechanisms.

Materials and methods

Models of AAI were induced by intragastric administration of ethanol (8 g/kg). Seventy-two Specific pathogen-free (SPF) male Kunming mice were randomly divided into six groups: normal group, positive control group, AEPH of low dosage (100 mg/kg) group, AEPH of medium dose (200 mg/kg) group, AEPH of high dosage (400 mg/kg) group and model group. The mice were treated with metadoxine (MTD, 500 mg/kg) and AEPH. Thirty minutes later, the normal group was given normal saline, while the other groups were given ethanol (i.g., 8 g/kg). The impact of AEPH was observed. In the same way, another seventy-two Kunming mice were randomly divided into six groups equally. The blood ethanol concentration at 0.5, 1, 1.5, 2, 3 and 6 h after ethanol intake was determined by way of gas chromatography. The activity of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) and microsomal ethanol oxidase (EO) in liver, and the concentration of β-endorphin (β-EP), leucine-enkephalin (LENK) in the brain were determined by enzyme-linked-immunosorbent serologic assay (ELISA).

Results

AEPH markedly prolonged alcohol tolerance time and shortened sober-up time after acute ethanol administration. AEPH decreased blood ethanol levels in six tests after ethanol intake. The 7-day survival rate of AEPH group was obviously superior to model group. AEPH increased the activities of ADH, ALDH, and decreased EO activity in liver. The crucial find was that AEPH markedly decreased β-EP and LENK concentration in the brain.

Conclusions

AEPH can markedly increase the levels of ADH, ALDH, decrease EO activity in liver and decrease the concentration of β-EP and LENK in the brain to against acute alcohol intoxication in mice.