Regulation of mitochondrial genome replication by hypoxia: The role of DNA oxidation in D-loop region
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- 作者：Viktor M. Pastukh vpastukh@southalabama.edu" class="auth_mail" title="E-mail the corresponding author ; Olena M. Gorodnya gorodnya@southalabama.edu" class="auth_mail" title="E-mail the corresponding author ; Mark N. Gillespie mgillesp@southalabama.edu" class="auth_mail" title="E-mail the corresponding author ; Mykhaylo V. Ruchko ; mruchko@southalabama.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ANOVA ; analysis of variance ; ATP6 ; ATP synthase subunit 6 ; BER ; base excision repair ; ChIP ; chromatin immunoprecipitation ; Cox2 ; cytochrome c oxidase subunit 2 ; DIG ; digoxigenin ; Fpg ; formamidopyrimidine DNA glycosylase ; mtDNA ; mitochondrial DNA ; ND4 ; NADH dehydrogenase subunit 4 ; Ogg1 ; 8-oxoguanine glycosylase ; PAECs ; pulmonary artery endothelial cells ; PBS ; phosphate-buffered saline ; ROS ; reactive oxygen species ; RT-PCR ; reverse transcription PCR ; SE ; standard error ; TE ; Tris-EDTA ; TFAM ; mitoc
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：78-88
- 全文大小：917 K
文摘
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A new mechanism, by which hypoxia triggers mtDNA replication, is proposed.
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Hypoxia-induced mtDNA oxidative modifications are prominent in the D-loop region.
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Such modifications are important for TFAM binding and subsequent mtDNA replication.
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Overexpression of Ogg1 prevents hypoxia-induced oxidation of the D-loop region.
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Ogg1 overexpression attenuates TFAM binding and mtDNA replication under hypoxia.
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