Second Harmonic Generation, a new approach for analyzing the interfacial properties of a short tryptophan-rich peptide

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• 作者：Gladys Matar ; Julien Duboisset ; Emmanuel Benichou ; Guillaume Bachelier ; Isabelle Russier-Antoine ; Christian Jonin ; Damien Ficheux ; Pierre-Franç ; ois Brevet ; Franç ; oise Besson
• 刊名：Chemical Physics Letters
• 出版年：2010
• 出版时间：10 November 2010
• 年：2010
• 卷：500
• 期：1-3
• 页码：161-166
• 全文大小：614 K

文摘

The involvement of the tryptophan residues in the interactions of peptides or proteins with lipid membranes justifies our characterization of the interfacial properties of a synthetic tryptophan-rich peptide by a new approach using the nonlinear optic properties of this amino acid. The positively-charged peptide (KWWKWWK) at pH 8.5, alone or in the presence of a negatively-charged phospholipid monolayer, was detected at the air–water interface by combined optical Second Harmonic Generation (SHG), a nonlinear spectroscopy, and surface tension measurements. Further polarized SHG experiments showed that the short peptide was oriented at the negatively-charged phospholipid interface.