Efficient secretory expression of recombinant proteins in Escherichia coli with a novel actinomycete signal peptide
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- 作者：Yanbing Cuia ; Yiwei Menga ; Juan Zhangb ; Bin Chengb ; Huijia Yina ; Chao Gaoa ; Ping Xua ; Chunyu Yanga ; ycy21th@sdu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Actinomycete signal peptide ; Extracellular expression ; Inducible and constitutive expression ; High rare codon content protein
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：69-74
- 全文大小：804 K
文摘
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A novel actinomycete signal peptide was effective in E. coli expression.
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Three hydrolases were secretory expressed by fusing with the signal peptide.
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Both inducible and constitutive systems were successfully constructed.
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