Colorimetric assay of l-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters

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• 作者：Liang-Liang WuAuthor Vitae ; Li-Ying WangAuthor Vitae ; Zheng-Jun XieAuthor Vitae ; Na PanAuthor Vitae ; Chi-Fang Peng ; ^{pcf@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Ag/Pt nanoclusters ; Enzyme mimic ; l-Cysteine ; Colorimetric detection
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：1 November 2016
• 年：2016
• 卷：235
• 期：Complete
• 页码：110-116
• 全文大小：2619 K

文摘

This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by l-cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for l-cysteine was developed with a detection limit (LOD) of 2.0 nM and a linear range from 5.0 nM to 500 nM. This detection for l-cysteine was highly selectivity over other amino acids. The high sensitivity and selectivity of this method facilitated the detection of l-cysteine in biological samples. Moreover, this method developed here was low-cost and simple. These results indicated the excellent analytical application of Ag/Pt NCs as enzyme mimic.