Phylogenetic analysis and prokaryotic expression of NMAAP1 derived from BCG-activated murine macrophages

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• 作者：Xiangfeng Zhao^a ; ^{zxf184@163.com} ; Linbing Zhang^a ; Dongmei Yan^a ; Xiuping Feng^a ; Dun Chu^a ; Bin Li^a ; Bairong Du^a ; Xun Zhu ; ^a ; ^{zxunzhux@vip.sohu.com}
• 关键词：NMAAP1 ; BCG-activated macrophages ; Prokaryotic expression ; Sequence and phylogenetic analysis
• 刊名：Comparative Immunology, Microbiology and Infectious Diseases
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：34
• 期：2
• 页码：123-128
• 全文大小：361 K

文摘

BCG-activated macrophages exerted anti-tumor activities. Cell surface molecules play an important role in mediating endocytosis by macrophages. In the previous study, we identified a group of 454 membrane proteins specifically expressed on BCG-activated mouse macrophages, including a protein named NMAAP1 (novel macrophage activated associated protein). In this study, we aligned the full-length nucleotide sequences of NMAAP1 and its homologous sequences to construct its phylogenetic tree, and cloned the NMAAP1 cDNA from BCG-activated macrophages to generated NMAAP1 fusion protein in Escherichia coli. Purified the fusion protein were applied for generation of polyclonal antibodies. Western-blotting detection showed that the polyclonal antibodies have high specificities to recognize target protein.