Cloning and characterization of the cDNA and promoter of UDP-glucose:flavonoid 3-O-glucosyltransferase gene from a purple-fleshed sweet potato
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- 作者：M. Hu1 ; Z. Lu1 ; J. Guo ; Y. Luo ; H. Li ; L. Li ; F. Gao ; peak0041@vip.sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：UF3GT ; UDP-glucose ; flavonoid 3-O-glucosyltransferase ; RACE ; rapid amplification of cDNA ends ; FR ; fibrous roots ; TR ; thick roots ; SR ; storage roots ; GUS ; beta-glucuronidase
- 刊名：South African Journal of Botany
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：106
- 期：Complete
- 页码：211-220
- 全文大小：1754 K
文摘
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The full-length cDNA sequence of IbUF3GT was cloned from purple-fleshed sweet potato.
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IbUF3GT expression profiles were associated with anthocyanin biosynthesis in sweet potato.
• IbUF3GT was a functional enzyme involved in anthocyanin biosynthesis base on complementation of Arabidopsis UF3GT mutation.
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A 758 bp promoter of IbUF3GT gene was cloned and the key promoter activity region was found between − 758 bp and − 633 bp.
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