Study of strains of Candida spp. Isolated from catheters in UHC of Oran (Algeria): Identification and antifungal susceptibility

详细信息    查看全文

• 作者：M. Bendjelloul^a ; ^{doc.meri@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; Z. Boucherit-Otmani^a ; ^{z_boucherit@yahoo.fr" class="auth_mail" title="E-mail the corresponding author} ; K. Boucherit^a ; ^b ; ^{boucheritkebir@yahoo.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Candida spp. ; Catheters ; Amphotericin B ; Caspofungin ; Nosocomial infections
• 刊名：Journal de Mycologie Médicale / Journal of Medical Mycology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：26
• 期：3
• 页码：212-216
• 全文大小：427 K

文摘

The increasing incidence of Candida spp., and the vital prognosis often compromise for patients with Candida species make urgent the exact knowledge of their distribution worldwide and exhaust action antifungals currently used in clinical. That why we carry out an epidemiological study of Candida species and testing their susceptibility against two antifungals: amphotericin B and caspofungin.

Materials and methods

Samplings of peripheral venous catheters (PVC) were carried out from during 8 months on the services of Internal medicine, Surgery A and Neonatology of Oran's University Hospital Center (UHC). The study of the susceptibility of Candida species to antifungal agents was performed according to the Clinical Laboratory Standards Institute (CLSI 2008).

Results

From 300 samples, 25 yeasts were isolated. The rate of colonization PVC was 8.33% by Candida spp. The most isolated strains were Candida parapsilosis with 64% of cases, followed by Candida albicans (12%) then 8% for Candida glabrata and Candida krusei. However, only 4% of isolates were Candida famata or Candida lusitaniae. Furthermore all isolated strains were susceptible to amphotericin B with Minimum Inhibitory Concentrations (MIC) ranging from 0.25 to 1 μg/mL. MIC obtained with caspofungin vary from 0.0625 to 2 μg/mL for all strains. Moreover, one strain of C. krusei is resistant to caspofungin with a MIC superior to 8 μg/mL.

Conclusion

All though caspofungin is at least as effective as amphotericin B, it is better tolerated for the treatment of invasive fungal infections.