Exploring the dynamics of progenitor cells in the urethra after simulated birth trauma in mice

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• 作者：Hung-Yen Chin<sup>asup><sup> ; sup><sup>bsup> ; Li-Hsuan Chiu<sup>asup> ; Kuan-Cheng Lin<sup>csup> ; Chi-Hsin Chiang<sup>bsup> ; Chin-Jung Wang<sup>dsup><sup> ; sup><sup>esup><sup> ; sup><sup>wang2260@gmail.com" class="auth_mail" title="E-mail the corresponding authorsup>
• 关键词：pelvic floor ; progenitor cells ; trauma ; urinary incontinence ; wound healing
• 刊名：Taiwanese Journal of Obstetrics and Gynecology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：55
• 期：5
• 页码：650-653
• 全文大小：946 K

文摘

To examine the alteration in the cellular dynamics of the urethral tissue after a simulated birth trauma in a mouse model.

sSec_2">Materials and Methods

spara0015">A total of 36 B6 mice received vaginal distention treatment, and four untreated mice were used as controls. Specimens were collected every 24 hours after the injury for 9 consecutive days and examined using immunofluorescent staining for cell markers including c-kit, smooth muscle actin (SMA), and vimentin. Confocal microscopy was used to localize the stained cells and determine the cell number.

sSec_3">Results

spara0020">The number of c-kit positive cells increased after the 1<sup>stsup> day and peaked on the 3<sup>rdsup> day. The amount of SMA positive cells rapidly reduced to its lowest count on the 1<sup>stsup> day and maintained a statistically significant low cell number than that at the basal level for 4 days after vaginal distension. The cell number finally returned to basal level on the 9<sup>thsup> day. The amount of vimentin positive cells increased dramatically after the 1<sup>stsup> day and plateaued from the 3<sup>rdsup> day to the 9<sup>thsup> day. The number of vimentin positive cells in the plateau phase was significantly higher than that of the control group.

sSec_4">Conclusion

spara0025">Our study confirmed that the dynamic change in different cell types after the urethral injury was dependent on the nature and physiology of the wound repairing cells during the tissue healing process. It might be a simple animal model to study birth trauma repair; however, the varied progenitor cell activity in different species should also be considered.