Thermodynamic of chymosin partition in macromolecule-salt ATPS was determined.

Transfer parameters (mmlsi1" class="mathmlsrc">mathImg" data-mathURL="/science?_ob=MathURL&_method=retrieve&_eid=1-s2.0-S0960308516300980&_mathId=si1.gif&_user=111111111&_pii=S0960308516300980&_rdoc=1&_issn=09603085&md5=a09025c2a42448644177afed91c73386">mg class="imgLazyJSB inlineImage" height="18" width="51" alt="View the MathML source" title="View the MathML source" src="/sd/grey_pxl.gif" data-inlimgeid="1-s2.0-S0960308516300980-si1.gif">mathContainer hidden">mathCode"><math altimg="si1.gif" overflow="scroll"><mrow><mi mathvariant="normal">Δmi><msubsup><mrow><mi>Gmi>mrow><mrow><mi>tmi><mi>rmi><mo>,mo><mi>Cmi><mi>hmi><mi>ymi>mrow><mo>∞mo>msubsup>mrow>math>, mmlsi2" class="mathmlsrc">mathImg" data-mathURL="/science?_ob=MathURL&_method=retrieve&_eid=1-s2.0-S0960308516300980&_mathId=si2.gif&_user=111111111&_pii=S0960308516300980&_rdoc=1&_issn=09603085&md5=650f83d88a03cea3deb86d87d4b1b876">mg class="imgLazyJSB inlineImage" height="18" width="51" alt="View the MathML source" title="View the MathML source" src="/sd/grey_pxl.gif" data-inlimgeid="1-s2.0-S0960308516300980-si2.gif">mathContainer hidden">mathCode"><math altimg="si2.gif" overflow="scroll"><mrow><mi mathvariant="normal">Δmi><msubsup><mrow><mi>Hmi>mrow><mrow><mi>tmi><mi>rmi><mo>,mo><mi>Cmi><mi>hmi><mi>ymi>mrow><mo>∞mo>msubsup>mrow>math> and mmlsi3" class="mathmlsrc">mathImg" data-mathURL="/science?_ob=MathURL&_method=retrieve&_eid=1-s2.0-S0960308516300980&_mathId=si3.gif&_user=111111111&_pii=S0960308516300980&_rdoc=1&_issn=09603085&md5=aed18326d15998e6957445c83d6f8a7c">mg class="imgLazyJSB inlineImage" height="18" width="49" alt="View the MathML source" title="View the MathML source" src="/sd/grey_pxl.gif" data-inlimgeid="1-s2.0-S0960308516300980-si3.gif">mathContainer hidden">mathCode"><math altimg="si3.gif" overflow="scroll"><mrow><mstyle mathvariant="normal"><mi mathvariant="normal">Δmi>mstyle><msubsup><mi>Smi><mrow><mi>tmi><mi>rmi><mo>,mo><mi>Cmi><mi>hmi><mi>ymi>mrow><mo>∞mo>msubsup>mrow>math>) were obtained by nanocalorimetry.

Chymosin transfer was enthalpically driven, with enthalpy-entropy compensation.

mmlsi6" class="mathmlsrc">mathImg" data-mathURL="/science?_ob=MathURL&_method=retrieve&_eid=1-s2.0-S0960308516300980&_mathId=si6.gif&_user=111111111&_pii=S0960308516300980&_rdoc=1&_issn=09603085&md5=38b827d5776fe6051206e970a42fae9c">mg class="imgLazyJSB inlineImage" height="18" width="55" alt="View the MathML source" title="View the MathML source" src="/sd/grey_pxl.gif" data-inlimgeid="1-s2.0-S0960308516300980-si6.gif">mathContainer hidden">mathCode"><math altimg="si6.gif" overflow="scroll"><mrow><mi mathvariant="normal">Δmi><msubsup><mrow><mi>Hmi>mrow><mrow><mi>dmi><mi>imi><mi>lmi><mo>,mo><mi>Cmi><mi>hmi><mi>ymi>mrow><mo>∞mo>msubsup>mrow>math> was used to ascertain the interactions involved in the enzyme partition.

Chymosin ATPS components interaction competition driven the enzyme partition.