This is the first large-scale proteomic data for barnyardgrass among the weeds in the crop fields.
Ecological fitness costs are associated with multiple-herbicide resistance in barnyardgrass.
Photosynthesis-related proteins are found to be down-accumulated in the resistant biotypes of barnyardgrass.
Protein species involved in plant defense are found to be up-accumulated in the resistant biotypes of barnyardgrass.
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