Molecular characterization and expression profiles of GATA6 in tongue sole (Cynoglossus semilaevis)

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• 作者：Jinxiang Liu ; Wei Zhang ; Yan Sun ; Zhigang Wang ; Quanqi Zhang ; Xubo Wang ; ^{wangxubo@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cynoglossus semilaevis ; GATA6 ; Dimorphic expression ; Gonad development ; Methylation
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：198
• 期：Complete
• 页码：19-26
• 全文大小：1755 K

文摘

GATA-binding protein 6 (GATA6), a transcription factor of the GATA family, plays an important role in gonadal cell proliferation, differentiation, and endoderm development. In this study, the full-length coding sequence of tongue sole (Cynoglossus semilaevis) GATA6 was identified. The sequence consisted of 1494 nucleotides encoding a peptide of 497 amino acids, which included two conserved zinc finger domains. Phylogenetic, gene structure, and synteny analysis showed that C. semilaevis GATA6 was homologous to teleost and tetrapod GATA6. C. semilaevis GATA6 mRNA exhibited high expression in heart, intestine, liver, kidney, and gonad. Embryonic development expression profiles revealed that GATA6 is involved in morphogenesis because its expression increased at the blastula stage. The in situ hybridization results showed strong GATA6 signals in spermatogonia, spermatocytes, and Sertoli cells of the testis. The signals were also detected in the oogonia and oocytes of the ovary. The expression of C. semilaevis GATA6 was sexually dimorphic, and the methylation pattern in the promoter region varied among males, females, and pseudomales. These results suggested that GATA6 might influence the gonad development and reproduction of C. semilaevis. This study provides the groundwork for further development of breeding techniques in C. semilaevis.