Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats

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• 作者：Ling Fan¹ ; ² ; Zhao Chen³ ; Wenbin Fu¹ ; ^{546624680@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Nenggui Xu⁴ ; Jianhua Liu¹ ; Aiping Lu⁵ ; Ziping Li¹ ; Shengyong Su⁶ ; Taixiang Wu⁷ ; Aihua Ou⁸
• 关键词：acupuncture ; antidepressant ; botulinum toxin A ; riluzole
• 刊名：Journal of Acupuncture and Meridian Studies
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：9
• 期：5
• 页码：242-249
• 全文大小：973 K

文摘

This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague–Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p > 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.