Self-assembly molecularly imprinted polymers of 17¦Â-estradiol on the surface of magnetic nanoparticles for selective separation and detection of estrogenic hormones in feeds

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• 作者：Shu Wang ; Yun Li ; Meijuan Ding ; Xiaoli Wu ; Jinhui Xu ; Ruoyu Wang ; Tingting Wen ; Wenyu Huang ; Ping Zhou ; Kunfang Ma ; Xuemin Zhou ; ^{xueminzhou001_001@yahoo.cn} ; Shuhu Du ; ^{shuhudu@njmu.edu.cn}
• 关键词：Self-assembly molecularly imprinted polymers ; Magnetic nanoparticles ; Dispersive solid-phase extraction ; 17¦Â-Estradiol ; Feeds
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2011
• 出版时间：1 September, 2011
• 年：2011
• 卷：879
• 期：25
• 页码：2595-2600
• 全文大小：1K

文摘

This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, ¦Ã-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17¦Â-estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation and fast enrichment of estrogens from the feeds. The assay exhibited a linear range of 0.1-4 ¦ÌM for E2 and estriol (E3) with the correlation coefficient above 0.9996 and 0.9994, respectively. Recoveries of E2 from three kinds of feeds spiked at different concentration levels ranged from 92.7 % to 97.0 % with RSD < 4.7 % , and recoveries of E3 ranged from 71.9 % to 83.1 % with RSD < 4.9 % , respectively. The method is simple and sensitive, and can be used as an alternative tool to effectively separate and enrich the trace of estrogens in agricultural products by HPLC-UV.