Screening method for the analysis of antiviral drugs in poultry tissues using zwitterionic hydrophilic interaction liquid chromatography/tandem mass spectrometry

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• 作者：D. Chan^a ; ^{danny.chan@fera.gsi.gov.uk"" rel=""nofollow} ; J. Tarbin^a ; M. Sharman^a ; M. Carson^b ; M. Smith^b ; S. Smith^b
• 关键词：Antiviral ; Amantadine ; Acyclovir ; Ganciclovir ; Imiquimod ; Oseltamivir ; Oseltamivir carboxylate ; Rimantadine ; Poultry ; Zanamivir
• 刊名：Analytica Chimica Acta
• 出版年：2011
• 出版时间：26 August 2011
• 年：2011
• 卷：700
• 期：1-2
• 页码：194-200
• 全文大小：654 K

文摘

A screening method for the analysis of seven anti-viral drugs in poultry tissue has been developed. These include anti-influenza drugs (amantadine, rimantadine, zanamivir and oseltamivir and its carboxylate metabolite), anti-herpes drugs (acyclovir and ganciclovir) and an immunomodulator (imiquimod). Poultry tissue was extracted in acetonitrile:water:acetic acid. After sample purification, using a strong cation exchange column, the eluate was split into two fractions. The first portion was dissolved in methanol:water and the second in acetonitrile:methanol:water. Both fractions were analysed on a zwitterionic hydrophilic interaction liquid chromatography column coupled to a triple quadrupole mass spectrometer. The screening method was successfully validated according to Commission Decision 2002/657/EC in three different laboratories with CCβ concentrations of ≤10 μg kg⁻¹.