Optimisation of extraction conditions for polysaccharides from the roots of Isatis tinctoria L. by response surface methodology and their in vitro free radicals scavenging activities and effects on IL-4 and IFN-γ mRNA expression in chicken lymphocyt

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• 作者：Jiangwei Han^a ; Xingming Jiang^b ; Lida Zhang^c ; ^{zhangld0808@yahoo.com.cn"" rel=""nofollow}
• 关键词：Response surface methodology ; Polysaccharides ; Isatis tinctoria L. ; Optimization ; Extraction ; Free radicals scavenging activities ; Chicken lymphocytes
• 刊名：Carbohydrate Polymers
• 出版年：2011
• 出版时间：30 August 2011
• 年：2011
• 卷：86
• 期：3
• 页码：1320-1326
• 全文大小：634 K

文摘

Response surface methodology along with Box-Behnken design was firstly applied to optimize the extraction conditions for the polysaccharides from the roots of Isatis tinctoria L. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. The 3-D response surface and the contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimal conditions were extraction temperature (X₁) 99.5 °C, extraction time (X₂) 3.75 h and ratio of water to raw material (X₃) 11.84 (v/w). Under these conditions, the maximal observed value extraction yield of Isatis root polysaccharides (IRPSs) was (11.19 ± 0.04) % , which was agreed with predicted value 11.17 % . Pharmacological experiments indicated that IRPS have an appreciable ABTS radical scavenging ability in vitro and could increase IL-4 and IFN-γ mRNA expression in chicken lymphocytes obtaining maximum promoted effects of 70 % and 115 % , respectively. This study may facilitate a deeper understanding of IRPS to provide theoretical references.