Nitrogen modifies NaCl toxicity in eggplant seedlings: Assessment of chlorophyll a fluorescence, antioxidative response and proline metabolism

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• 作者：Madhulika Singh ; Vijay Pratap Singh¹ ; ^{vijaypratap.au@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Sheo Mohan Prasad ; ^{profsmprasad@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ABS/RC ; the energy fluxes for absorption of photon per active RC ; CAT ; catalase ; DI0/RC ; energy dissipation flux per active RC ; ET0/RC ; electron transport flux per active RC ; Fv/F0 ; size and number of active reaction center of photosynthetic apparatus ; F0/Fv ; Efficiency of water splitting complex ; Fv/Fm or Phi_P0 or ϕP0 ; quantum yield for primary photochemistry ; GST ; glutathione-S-transferase ; H2O2 ; hydrogen peroxide ; K ; potassium ; MDA ; malondialdehyde ; N ; nitrogen ; Na ; sodium ; NaCl ; sodium chlo
• 刊名：Biocatalysis and Agricultural Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：76-86
• 全文大小：1659 K

文摘

In the present study, effect of different levels [0, 25,75 and 150 kg NO₃⁻ ha⁻¹ sand correspond to N₀ (deprived), N₂₅ (sub-optimum), N₇₅ (optimum) and N₁₅₀ (supra-optimum)] of nitrate in eggplant (Solanum melongena L.) seedlings under NaCl (NaCl₁, 0.3 g kg⁻¹ sand and NaCl₂, 0.5 g kg⁻¹ sand, respectively) stress was investigated. Growth, photosynthetic pigments and K⁺ content in test plant were declined by both the doses of NaCl. Furthermore, fluorescence parameters (JIP-test): F_m/F₀, F_v/F₀, F_v/F_m or ϕP_0, ϕE₀, Ψ₀, PI_ABS, ABS/RC, TR₀/RC, ET₀/RC and DI₀/RC were also affected by NaCl but toxic effects of NaCl on photosystem II photochemistry were ameliorated by N. NaCl increased accumulation of Na⁺ and oxidative stress biomarkers: superoxide radical, hydrogen peroxide, lipid peroxidation and electrolyte leakage despite increased activities of superoxide dismutase, peroxidase, catalase and glutathione-S-transferase. N addition caused enhancement in NaCl-mediated decline in endogenous proline and activity of Δ₁-pyrroline-5-carboxylate synthetase (P5CS), while the activity of proline dehydrogenase decreased. The results indicate that different levels of N significantly modulated NaCl-induced damaging effects in eggplant. Further, results suggest that after N addition Na⁺ content, enzymatic antioxidants, and pool of free proline and activity of P5CS are finely regulated, which might be associated with the mitigation of NaCl stress and this effect was more pronounced with supra-optimum level of N.