A target-triggered dual amplification strategy for sensitive detection of microRNA
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- 作者：Weifeng Lva ; b ; Jiamin Zhaoa ; b ; Bo Situa ; b ; Bo Lia ; b ; Wen Maa ; b ; Jumei Liua ; b ; Zixian Wua ; b ; Wen Wangc ; Xiaohui Yand ; Lei Zhenga ; b ; nfyyzhenglei@smu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：MicroRNA ; Duplex-specific nuclease ; Chimeric probes ; Dual signal amplification ; Magnetic microspheres
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：83
- 期：Complete
- 页码：250-255
- 全文大小：1447 K
文摘
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A novel fluorescence biosensor for highly sensitive and selective detection of miRNA was established.
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A dual signal amplification strategy based on single enzyme (DSN) was developed and evaluated.
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DNA/2-OMe-RNA chimeric probe was utilized to connect two rounds of cyclical amplification.
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This strategy can easily be applied to all miRNAs and holds great potential for further application.
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