Carbon nanospheres enhanced electrochemiluminescence of CdS quantum dots for biosensing of hypoxanthine

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• 作者：Yangyang  ; Zhang ; Shengyuan  ; Deng ; Jianping  ; Lei ; Qiunan  ; Xu ; Huangxian  ; Ju  ; ;   ; ^{hxju@nju.edu.cn}
• 关键词：Biosensors ; Electrochemiluminescence ; Quantum dots ; Carbon nanospheres ; Hypoxanthine
• 刊名：Talanta
• 出版年：2011
• 出版时间：30 September 2011
• 年：2011
• 卷：85
• 期：4
• 页码：2154-2158
• 全文大小：585 K

文摘

This work developed a novel method to greatly enhance the electrochemiluminescence (ECL) of CdS quantum dots (QDs). The ECL amplification was achieved by the assembly of QDs on poly (diallyldimethylammonium chloride)-functionalized carbon nanospheres (PFCNSs), and successfully employed for sensitive ECL biosensing of oxidase substrates. The carbon nanospheres were prepared by a ¡°green?method, and the high loading of QDs on carbon nanospheres led to a 4-times increased ECL intensity with dissolved O₂ as the coreactant. Using xanthine oxidase (XOD) as a model, an ECL biosensor was fabricated by immobilizing the enzyme on the mixing membrane of PFCNSs and QDs. The ECL biosensor showed a fast response to hypoxanthine with a linear concentration range from 2.5 ¡Á 10^? to 1.4 ¡Á 10^? M. The limit of detection was 5 nM at a signal-to-noise ratio of 3. The assay results of hypoxanthine in fish samples were in a good agreement with the reference values by amperometric technique. This facile approach to prepare the PFCNSs/QDs system for ECL biosensing could be of promising application in bioanalysis and electronic device.