Investigation of VUV photodissociation propensities using peptide libraries

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• 作者：Xiaohui  ; Liu^a ; Yong Fuga  ; Li^b ; Brian C.  ; Bohrer^a ; Randy J.  ; Arnold^a ; Predrag  ; Radivojac^b ; Haixu  ; Tang^b ; James P.  ; Reilly^a ;   ; ^{reilly@indiana.edu}
• 关键词：De novo sequencing ; Photodissociation ; Mass spectrometry ; MALDI-TOF/TOF
• 刊名：International Journal of Mass Spectrometry
• 出版年：2011
• 出版时间：1 December 2011
• 年：2011
• 卷：308
• 期：2-3
• 页码：142-154
• 全文大小：1681 K

文摘

PSD does not usually generate a complete series of y-type ions, particularly at high mass, and this is a limitation for de novo sequencing algorithms. It is demonstrated that b₂ and b₃ ions can be used to help assign high mass x_N? and x_N? fragments that are found in vacuum ultraviolet (VUV) photofragmentation experiments. In addition, v_N-type ion fragments with side chain loss from the N-terminal residue often enable confirmation of N-terminal amino acids. Libraries containing several thousand peptides were examined using photodissociation in a MALDI-TOF/TOF instrument. 1345 photodissociation spectra with a high S/N ratio were interpreted.