Lewisite metabolites detection in urine by liquid chromatography¨Ctandem mass spectrometry

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• 作者：Igor Rodin^a ;   ; ^{rodin@analyt.chem.msu.ru} ; Arkady Braun^a ;   ; ¹ ;   ; ^{avbraun@yandex.ru} ; Andrey Stavrianidi^a ;   ; ¹ ;   ; ^{didi007forall@mail.ru} ; Oleg Shpigun^a ;   ; ² ;   ; ^{shpigun@analyt.chem.msu.ru} ; Igor Rybalchenko^b ;   ; ³ ;   ; ^{Riv@lumex.msk.ru}
• 关键词：Lewisite ; Liquid chromatography ; Tandem mass spectrometry
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2011
• 出版时间：15 December 2011
• 年：2011
• 卷：879
• 期：32
• 页码：3788-3796
• 全文大小：1690 K

文摘

A sensitive and simple method for the quantification and for the detection of 2-chlorovinylarsonous (CVAA) and 2-chlorovinylarsonic (CVAOA) acids was developed. CVAA and CVOA are important biological markers in human and rat urine specific to lewisite (chlorovinylarsonous chloride compounds) exposure. The developed assay was based on the use of solid-phase extraction (SPE) followed by liquid-chromatography coupled to electrospray ionization (negative ion-mode) low-energy collision dissociation-tandem mass spectrometry (ESI-CID-MS/MS). The method demonstrated linearity over at least three orders of magnitude and had a detection limit (LOD) of 0.5 ng/ml for CVAA and 3 ng/ml for CVAOA. The relative standard deviations for the quality control samples ranged from 6 to 11 % . Application of this procedure was demonstrated in the lewisite animals exposure model. Rats were exposed intravenously by no lethal doses of lewisite and markers levels in urine samples were analyzed for 21 days post-exposure.