Characterization of a new acidic NAD+-dependent formate dehydrogenase from thermophilic fungus Chaetomium thermophilum
详细信息 查看全文
- 作者：Gü ; lşah Ö ; zgü ; na ; b ; Nevin Gü ; l Karagü ; lera ; b ; Ossi Turunenc ; Nicholas J. Turnerd ; Barış Binaye ; binaybaris@gmail.com" class="auth_mail" title="E-mail the corresponding author ; barisbinay@arel.edu.tr" class="auth_mail" title="E-mail the corresponding author
- 关键词：NAD+-dependent acidic formate dehydrogenase ; Chaetomium thermophilum ; Thermostability ; Biochemical and kinetic characterization ; Computational modeling
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：122
- 期：Complete
- 页码：212-217
- 全文大小：812 K
文摘
- •
Chaetomium thermophilum formate dehydrogenase was overexpressed in E. coli.
- •
The pH optimum was at pH 5, which is unusual for formate dehydrogenases.
- •
The enzyme showed moderate thermostability and considerable storage stability at +4 °C.
- •
Molecular properties of dimer–dimer interface were analyzed by modelling.
- •
NAD+-mediated metal-independent hydride shift appears to be the reaction mechanism.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |